Promega Corporation

CYP1A2 Assay Systems

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The P450-Glo™ CYP450 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 activity. The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. These luminescent assays exhibit exquisite sensitivity, low background signals and broad dynamic range.

P450-Glo™ Assays employ luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to...

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  • Prices valid for customers of Promega France only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

P450-Glo™ CYP1A2 Induction/Inhibition Assay

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P450-Glo™ CYP1A2 Induction/Inhibition Assay

 Components

  • Luciferin-1A2

    V841A1 x 30μl
  • D-Cysteine, 500X

    V843A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
  • Reconstitution Buffer

    V865A1 x 10ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10ml
- V8421 € 155,00 Add to cart

P450-Glo™ CYP1A2 Induction/Inhibition Assay

Close Window

P450-Glo™ CYP1A2 Induction/Inhibition Assay

 Components

  • Luciferin-1A2

    V841A2 x 30μl
  • D-Cysteine, 500X

    V843A1 x 100μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Reconstitution Buffer

    V865B1 x 50ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50ml
- V8422 € 618,00 Add to cart

P450-Glo™ CYP1A2 Assay

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P450-Glo™ CYP1A2 Assay

 Components

  • Luciferin Detection Reagent

    V859A1 x 1 each
  • Luciferin-ME, 5mM

    V862A1 x 200μl
  • Reconstitution Buffer

    V865A1 x 10ml
10ml
- V8771 € 155,00 Add to cart

P450-Glo™ CYP1A2 Assay

Close Window

P450-Glo™ CYP1A2 Assay

 Components

  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Luciferin-ME, 5mM

    V862B1 x 1ml
  • Reconstitution Buffer

    V865B1 x 50ml
50ml
- V8772 € 618,00 Add to cart

P450-Glo™ CYP1A2 Screening System

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P450-Glo™ CYP1A2 Screening System

 Components

  • Luciferin-Free Water

    V348A1 x 50ml
  • Potassium Phosphate Buffer, 1M

    V446A1 x 5ml
  • Control Membranes

    V476A1 x 100μl
  • CYP1A2 (1pmol/μl) + Reductase

    V477A1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Luciferin-ME, 5mM

    V862B1 x 1ml
  • Reconstitution Buffer

    V865B1 x 50ml
  • Solution A—NADPH Regeneration System

    V952A1 x 2.75ml
  • Solution B—NADPH Regeneration System

    V953A1 x 600μl
1,000 assays
- V9770 € 863,00 Add to cart


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Storage Conditions

Store the CYP1A2 membranes at –70°C. Cytochrome P450 may lose activity with repeated freeze-thaw cycles. Avoid multiple freeze-thaw cycles by dispensing the CYP1A2 membranes into single-use aliquots (e.g., 50μl for 96 reactions). Store aliquots at –70°C. All other components can be stored at –20°C or –70°C and protected from light.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V8421, V8422, V8771, V8772, V9770 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V8421, V8422, V8771, V8772, V9770 U.S. Pat. No. 7,692,022 and other patents pending.

V8421, V8422, V8771, V8772, V9770 Patent Pending.

V8421, V8422, V8771, V8772, V9770 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V8421, V8422, V8771, V8772, V9770 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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