Promega Corporation

DeadEnd™ Colorimetric TUNEL System

The DeadEnd™ Colorimetric TUNEL System is a modified TUNEL Assay designed to provide simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level. The DeadEnd™ Colorimetric TUNEL System measures nuclear DNA fragmentation, an important biochemical indicator of apoptosis. The system can be used to assay apoptotic cell death in both tissue sections and cultured cells. The DeadEnd™ Colorimetric TUNEL System end-labels the fragmented DNA of apoptotic cells using a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Biotinylated nucleotide is incorporated at the 3´...

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  • Prices valid for customers of Promega France only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

DeadEnd™ Colorimetric TUNEL System

4.00
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DeadEnd™ Colorimetric TUNEL System

 Components

  • Plastic Coverslips

    G326B1 x 20 each
  • Equilibration Buffer

    G327B1 x 4.8ml
  • SSC, 20X

    G329B1 x 20ml
  • Streptavidin HRP

    G714A1 x 40μl
  • Biotinylated Nucleotide Mix

    G715A1 x 20μl
  • DAB 20X Chromogen

    G716A1 x 200μl
  • DAB Substrate 20X Buffer

    G717A1 x 200μl
  • Hydrogen Peroxide 20X

    G718A1 x 200μl
  • Terminal Deoxynucleotidyl Transferase (recombinant)

    M828B1 x 600u
  • Proteinase K

    V302A1 x 10mg
20 reactions
- G7360 € 359,00 Add to cart

DeadEnd™ Colorimetric TUNEL System

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DeadEnd™ Colorimetric TUNEL System

 Components

  • Plastic Coverslips

    G326B2 x 20 each
  • Equilibration Buffer

    G327C1 x 9.6ml
  • SSC, 20X

    G329A1 x 70ml
  • Streptavidin HRP

    G714A1 x 40μl
  • Biotinylated Nucleotide Mix

    G715A2 x 20μl
  • DAB 20X Chromogen

    G716A1 x 200μl
  • DAB Substrate 20X Buffer

    G717A1 x 200μl
  • Hydrogen Peroxide 20X

    G718A1 x 200μl
  • Terminal Deoxynucleotidyl Transferase (recombinant)

    M828B2 x 600u
  • Proteinase K

    V302A1 x 10mg
40 reactions
- G7130 € 530,00 Add to cart


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Storage Conditions

Store the Equilibration Buffer, TdT Enzyme, Biotinylated Nucleotide Mix and Proteinase K at –20°C. Store the Streptavidin HRP, DAB 20X Chromogen, DAB Substrate 20X Buffer and Hydrogen Peroxide 20X at 4°C. Store the SSC 20X and Plastic Coverslips at room temperature.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

G7360, G7130 For Research Use Only. Not for Use in Diagnostic Procedures.

DeadEnd Colorimetric TUNEL System

4.00

mercredi 20 juin 2012 by victoria moreno, CIPF, Valencia

protocolo no muy evidente, algo complicado, pero funciona bien

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