Promega Corporation

CytoTox 96® Non-Radioactive Cytotoxicity Assay

The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The CytoTox 96® Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected usi...

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CytoTox 96® Non-Radioactive Cytotoxicity Assay

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CytoTox 96® Non-Radioactive Cytotoxicity Assay


  • Substrate Mix

    G179A5 x 1.5ml
  • Assay Buffer

    G180A1 x 60ml
  • LDH Positive Control

    G181A1 x 25μl
  • Lysis Solution, 10X

    G182A1 x 5ml
  • Stop Solution

    G183A1 x 65ml
1,000 assays
- G1780 € 298,00 Add to cart

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Storage Conditions

Store Substrate Mix and Assay Buffer at –20°C. Store LDH Positive Control, Lysis Solution (10X) and Stop Solution at 4°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

G1780 For Research Use Only. Not for Use in Diagnostic Procedures.

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