T7 RNA Polymerase

T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis.

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T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis.

Features - Benefits

• Specific: T7 RNA Polymerase exhibits extremely high affinity and specificity for T7 promoter sequences.
• Highly Pure: T7 RNA Polymerase is judged to be greater than 90% pure as determined by SDS polyacrylamide gel electrophoresis. Free of detectable levels of contaminating RNase and DNase activity (<1% release).
• Flexible: Will incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates.
• Provided with 5X Reaction Buffer: Provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl₂, 10mM spermidine, 50mM NaCl.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Generation of templates for in vitro translation.
• Generation of probes for nucleic acid hybridizations.
• Generation of RNA processing substrates.
• Generation of antisense RNA.

References

1. Milligan, J.F. et al. (1987) Nucl. Acids. Res. 15, 8783–98.
2. Milligan, J.F. and Uhlenbeck. O.C. (1989) Meth. Enzymol. 180, 51–62.

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