Fixed-Tissue Genomic DNA Purification

The MagneSil® Genomic, Fixed Tissue System provides a fast, simple technique to prepare genomic DNA from formalin-fixed, paraffin-embedded tissue. After an overnight Proteinase K digestion, genomic DNA can be manually purified from formalin-fixed, paraffin-embedded thin tissue sections in less than an hour. Amplifiable genomic DNA can be isolated from 10μm thin sections without centrifugation of the lysate prior to purification. Up to 12 samples can be processed in the manual format using the MagneSphere® Technology Magnetic Separation Stand (twelve-position) (Cat.# Z5342).

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The MagneSil® Genomic, Fixed Tissue System provides a fast, simple technique to prepare genomic DNA from formalin-fixed, paraffin-embedded tissue. After an overnight Proteinase K digestion, genomic DNA can be manually purified from formalin-fixed, paraffin-embedded thin tissue sections in less than an hour. Amplifiable genomic DNA can be isolated from 10μm thin sections without centrifugation of the lysate prior to purification. Up to 12 samples can be processed in the manual format using the MagneSphere® Technology Magnetic Separation Stand (twelve-position) (Cat.# Z5342).

Features - Benefits

• Purify High-Quality DNA: The composition of the wash buffers and protocol have been optimized to yield genomic DNA that is largely free of small DNAs, a potent inhibitor of PCR amplification.
• Rely on Performance-Tested Amplification Results: Amplify targets in multiplex PCR and targets as large as 450–1,800bp.

Applications

For more information, see the Protocols & Applications Guide.

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