TMB One Solution

TMB One Solution is a chromagen substrate, 3,3´,5,5´-tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a single solution at a ready-to-use working dilution. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user.

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TMB One Solution is a chromagen substrate, 3,3´,5,5´-tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a single solution at a ready-to-use working dilution. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user.

Features - Benefits

• Convenient: Single solution provided ready-to-use; just add, incubate, stop and read. This homogeneous reagent improves assay variation.
• Stable: Stable for 12 months at 4°C, providing extended shelf life; the assay end product is stable for at least one hour after stopping the assay.
• Safe: Provided in a slightly acidic, nonhazardous proprietary buffer without aprotic solvents; noncaustic to plastics used in automated systems.
• Sensitive: Low background provides greater assay sensitivity.

Applications

• ELISA.

Note: Not recommended for Western analysis or immunohistochemistry.

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