Promega Corporation

pGL4 in vivo Imaging Vectors

See All pGL4 Vectors
The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available. The pGL4.50 and pGL4.51 Vectors offer the synthetic firefly luciferase luc2 gene under the control of the strong constitutive CMV (cytomegalovirus) promoter. These vectors have demonstrated high expression levels in a variety of cell lines tested. The addition of a selectable marker, either hygromycin or neomycin, also allows the creation of stable cell lines. Cell lines with constant expressi...

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pGL4.50[luc2/CMV/Hygro] Vector

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pGL4.50[luc2/CMV/Hygro] Vector

 Components

  • pGL4.50[luc2/CMV/Hygro] Vector

    E131A1 x 20μg
20μg
- E1310 € 421,00 Add to cart

pGL4.51[luc2/CMV/Neo] Vector

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pGL4.51[luc2/CMV/Neo] Vector

 Components

  • pGL4.51[luc2/CMV/Neo] Vector

    E132A1 x 20μg
20μg
- E1320 € 421,00 Add to cart


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GenBank®/EMBL Accession Number

pGL4.50[luc2/CMV/Hygro] Accession Number EU921840; pGL4.50[luc2/CMV/Hygro] sequence text file.
pGL4.51[luc2/CMV/Neo] Accession Number  EU921841; pGL4.51[luc2/CMV/Neo] sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

pGL4.50[luc2/CMV/Hygro] Vector.Figure 1. pGL4.50[luc2/CMV/Hygro] Vector.
pGL4.51[luc2/CMV/Neo] Vector. Figure 2. pGL4.51[luc2/CMV/Neo] Vector.

Use Restrictions

E1310, E1320 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E1310, E1320 The use of this product and derivatives thereof is strictly limited to that of a life sciences research reagent. All other use is strictly prohibited, including but not limited to any diagnostic, therapeutic, or commercial use. The use of this product for in vivo bioluminescent imaging may be covered by one or more patents controlled by Caliper Life Sciences, Inc. Those patents include U.S. Pat. Nos. 5,650,135, 6,217,847, 6,923,951, 6,908,605, 6,890,515, and corresponding foreign patents and pending applications. The purchase or transfer of this product is not intended, either expressly or by implication, to grant any right or license to practice under the foregoing patents. Purchasers are advised to contact Caliper Life Sciences, Inc., 68 Elm St., Hopkinton, MA 01748 for information regarding a license to practice under such patents.

E1310, E1320 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

E1310, E1320 U.S. Pat. No. 5,670,356.

E1310, E1320 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

E1310, E1320 U.S. Pat. No. 7,728,118.

E1310, E1320 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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