M-MLV Reverse Transcriptase
RNA-Dependent DNA Polymerase for cDNA Synthesis
- Enzyme supplied at a concentration of 200u/μl
Catalog Number:
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Catalog Number: M1701
Catalog Number: M1705
Catalog Number: M5313
RNA-Dependent DNA Polymerase for cDNA Synthesis
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Advantages- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
- May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
- Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Size |
|---|---|---|
|
M-MLV Reverse Transcriptase |
M170A | 1 × 10,000u |
|
M-MLV RT 5X Buffer |
M531A | 1 × 1ml |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box
| Item | Part # | Size |
|---|---|---|
|
M-MLV Reverse Transcriptase |
M170B | 1 × 50,000u |
|
M-MLV RT 5X Buffer |
M531A | 2 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box
| Item | Part # | Size |
|---|---|---|
|
M-MLV RT 5X Buffer |
M531A | 2 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
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