Promega Corporation

simplyRNA kits for the Maxwell® 16 Instrument

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The Maxwell® 16 simplyRNA Kits allow easy, automated RNA purification from cells, tissues and blood providing consistent yields of high-quality RNA for use in downstream applications.

  • Overview
  • Tissues & Cells
  • Blood
  • Simplified RNA Extraction: Reduce sample handling steps and avoid complicated instrument setup.
  • Less DNA Contamination: Higher purity than manual extraction and competitive products.
  • Reliable Performance: Prevent repeat purifications and failed experiments with consistent high-quality RNA for RT-qPCR.

Cost Savings

  • Complete, all-in-one kits provide all you need
  • All reagents are included in the kit, including DNase
  • Save by not repeating purifications and failed experiments
  • No additional costs for instrument methods or software
  • Experience lower total cost of ownership compared to QIAcube® instrument
9998TA
10000TA

Comparison of plastic kit consumables used to
process six (6) RNA sample.

Less Space

  • Compact solution for automating your sample extractions (less benchtop space)
  • Less plastic consumables per kit minimizes storage space

Less Waste

  • Fewer plastic consumables in kit compared to other automated commercial kits
  • Minimize plastic waste for disposal by using less plastic labware per process

Maxwell is a registered trademark of Promega Corporation.
QIAcube and RNeasy are registered trademarks of QIAGEN Corporation.
TRIzol is a registered trademark of Molecular Research Center, Inc.
PAXgene is a registered trademark of PreAnalytiX GmbH.

Automate RNA extraction from tissues or cells for 1–16 samples in <1hour

simplyRNA Protocol Overview

simplyRNA Protocol Overview

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Simplified RNA Extraction

  • Reduce pre-extraction handling to 4 steps
  • Extract RNA from 16 samples in <1 hour using paramagnetic-particle processing on the Maxwell® 16 instrument
  • Avoid complex and time consuming instrument setup associated with the QIAcube® instrument
  • No centrifugation required
  • No beta-mercaptoethanol (BME) required for sample preparation
  • No need to purchase extra items-all consumables included in the kit
  • Color coded reagent for DNase addition make it easy to find the correct well

Reliable Performance

  • Minimize variability from person to person with automation
  • Achieve improved RNA purity and consistent
  • Purify RNA for RT-PCR from low cell number and difficult tissues
  • Avoid repeat purifications and failed experiments
  • Keep RNA intact and contamination-free with minimal sample handling
  • Extract RNA from fresh or frozen cells
  • Automated particle processing eliminates liquid handling instrument clogging and drips

Intact RNA Extracted from Tissue Using the Maxwell® 16 LEV simplyRNA Tissue Kit

Figure 1. Extracted tissue samples were run on a FlashGel® System for 5 minutes and signal developed for 15 minutes. The 28S, 18S and 5S are clearly visible indicating intact RNA.

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Figure 1. Extracted tissue samples were run on a FlashGel® System for 5 minutes and signal developed for 15 minutes. The 28S, 18S and 5S are clearly visible indicating intact RNA.

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High Yield and Purity from Tissues

Works well with low-yield (e.g., heart) and high-yield (e.g., liver) tissues.

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Works well with low-yield (e.g., heart) and high-yield (e.g., liver) tissues.

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Improved RNA purity and reproducibility over manual extraction

Automated Maxwell 16 vs. Manual Trizol extraction.

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Automated Maxwell 16 vs. Manual Trizol extraction.

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More Sensitive

Average Cq determination from 100ng of total RNA.

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Average Cq determination from 100ng of total RNA.

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Less Contamination

Amplification of human Beta-microglobulin from equal volumes of eluate.

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Amplification of human Beta-microglobulin from equal volumes of eluate.

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Maxwell is a registered trademark of Promega Corporation.
QIAcube and RNeasy are registered trademarks of QIAGEN Corporation.
TRIzol is a registered trademark of Molecular Research Center, Inc.
PAXgene is a registered trademark of PreAnalytiX GmbH.

Automated RNA extraction from fresh whole blood for 1–16 samples in about an hour

Simplified RNA Extraction

  • Minimize sample handling before extraction
  • Extract RNA from 16 samples in about an hour using paramagnetic-particle processing on the Maxwell® 16 Instrument
  • Lower cost per preparation with no expensive collection tubes required
  • Fewer centrifuge steps than other systems
  • Use fresh whole blood in either EDTA or PaxGene tubes
  • Color coded reagents for DNase addition make it easy to find the correct well

Intact RNA with 28S, 18S and 5S rRNA bands visible

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Reliable Performance

  • Achieve improved yield and purity
  • Minimize DNA contamination
  • Obtain highly concentrated RNA from 2.5ml of whole blood
  • Purified RNA ready for down stream applications such as RT-PCR
  • Minimize variability from person to person with automation
  • Avoid repeat purifications and failed experiments
  • Automated particle processing eliminate clogging and drips of the liquid handling instrument
  • Obtain purified, intact RNA

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Maxwell is a registered trademark of Promega Corporation.
QIAcube and RNeasy are registered trademarks of QIAGEN Corporation.
TRIzol is a registered trademark of Molecular Research Center, Inc.
PAXgene is a registered trademark of PreAnalytiX GmbH.

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