Promega Corporation

TnT® Coupled Reticulocyte Lysate Systems

The TnT® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic cell-free protein expression: a single-tube, coupled transcription/translation system. The TnT® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard rabbit reticulocyte lysate translations commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters and require three separate reactions with several steps between each reaction. The TnT® Systems bypass many of these steps by incorporating transcription directly in the translatio...

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  • Prices valid for customers of Promega France only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

TnT® SP6 Coupled Reticulocyte Lysate System

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TnT® SP6 Coupled Reticulocyte Lysate System

 Components

  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • TnT® SP6 Polymerase

    L463A1 x 60μl
  • Luciferase SP6 Control DNA

    L474A1 x 5μg
  • TnT® Rabbit Reticulocyte Lysate

    L483A5 x 200μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
  • Magnesium Acetate

    L458A1 x 100μl
40 reactions
- L4600 € 644,00 Add to cart

TnT® SP6 Coupled Reticulocyte Lysate System, Trial Size

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TnT® SP6 Coupled Reticulocyte Lysate System, Trial Size

 Components

  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462B1 x 20μl
  • TnT® SP6 Polymerase

    L463C1 x 20μl
  • Luciferase SP6 Control DNA

    L474A1 x 5μg
  • TnT® Rabbit Reticulocyte Lysate

    L483A1 x 200μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Amino Acid Mixture Minus Leucine

    L995D1 x 25μl
  • Amino Acid Mixture Minus Methionine

    L996D1 x 25μl
  • Magnesium Acetate

    L458A1 x 100μl
8 reactions
- L4601 € 157,00 Add to cart

TnT® T7 Coupled Reticulocyte Lysate System

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TnT® T7 Coupled Reticulocyte Lysate System

 Components

  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • TnT® T7 Polymerase

    L481A1 x 60μl
  • Luciferase T7 Control DNA

    L482A1 x 5μg
  • TnT® Rabbit Reticulocyte Lysate

    L483A5 x 200μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
40 reactions
- L4610 € 644,00 Add to cart

TnT® T7 Coupled Reticulocyte Lysate System, Trial Size

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TnT® T7 Coupled Reticulocyte Lysate System, Trial Size

 Components

  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462B1 x 20μl
  • TnT® T7 Polymerase

    L481C1 x 20μl
  • Luciferase T7 Control DNA

    L482A1 x 5μg
  • TnT® Rabbit Reticulocyte Lysate

    L483A1 x 200μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Amino Acid Mixture Minus Leucine

    L995D1 x 25μl
  • Amino Acid Mixture Minus Methionine

    L996D1 x 25μl
8 reactions
- L4611 € 164,00 Add to cart

TnT® T3 Coupled Reticulocyte Lysate System

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TnT® T3 Coupled Reticulocyte Lysate System

 Components

  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • TnT® Rabbit Reticulocyte Lysate

    L483A5 x 200μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • TnT® T3 Polymerase

    L493A1 x 60μl
  • Luciferase T3 Control DNA

    L494A1 x 5μg
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
40 reactions
- L4950 € 644,00 Add to cart

TnT® T7/T3 Coupled Reticulocyte Lysate System

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TnT® T7/T3 Coupled Reticulocyte Lysate System

 Components

  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • TnT® T7 Polymerase

    L481B1 x 30μl
  • Luciferase T7 Control DNA

    L482A1 x 5μg
  • TnT® Rabbit Reticulocyte Lysate

    L483A5 x 200μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • TnT® T3 Polymerase

    L493B1 x 30μl
  • Luciferase T3 Control DNA

    L494A1 x 5μg
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
40 reactions
- L5010 € 645,00 Add to cart

TnT® T7/SP6 Coupled Reticulocyte Lysate System

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TnT® T7/SP6 Coupled Reticulocyte Lysate System

 Components

  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • TnT® SP6 Polymerase

    L463B1 x 30μl
  • Luciferase SP6 Control DNA

    L474A1 x 5μg
  • TnT® T7 Polymerase

    L481B1 x 30μl
  • Luciferase T7 Control DNA

    L482A1 x 5μg
  • TnT® Rabbit Reticulocyte Lysate

    L483A5 x 200μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
40 reactions
- L5020 € 645,00 Add to cart


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Storage Conditions

Store the polymerase at –20 to –70°C. Store Luciferase Assay Wells at room temperature. Store the other components at –70°C. Do not freeze-thaw the lysate more than two times.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

L4600, L4601, L4610, L4611, L4950, L5010, L5020 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L4600, L4601, L4610, L4611, L4950, L5010, L5020 U.S. Pat. Nos. 5,641,641 and 5,650,289.

L4600, L4601, L4610, L4611, L4950, L5010, L5020 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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