MagZ™ Protein Purification System

The MagZ™ Protein Purification System provides a simple, rapid and reliable method for the purification of expressed polyhistidine- or HQ-tagged proteins, which are 99% free of hemoglobin contamination, from rabbit reticulocyte lysate. Based on the use of proprietary, paramagnetic precharged particles, polyhistidine- or HQ-tagged protein can be isolated from 50–500μl of TnT® Coupled Transcription/Translation reactions. Polyhistidine- or HQ-tagged proteins bind to the particles in minutes, while unbound proteins are washed away, and the target protein is eluted with imidazole.

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The MagZ™ Protein Purification System provides a simple, rapid and reliable method for the purification of expressed polyhistidine- or HQ-tagged proteins, which are 99% free of hemoglobin contamination, from rabbit reticulocyte lysate. Based on the use of proprietary, paramagnetic precharged particles, polyhistidine- or HQ-tagged protein can be isolated from 50–500μl of TnT® Coupled Transcription/Translation reactions. Polyhistidine- or HQ-tagged proteins bind to the particles in minutes, while unbound proteins are washed away, and the target protein is eluted with imidazole.

Features - Benefits

• Specific: Minimal hemoglobin (<0.1%) binding to the MagZ™ Binding Particles.
• Quick: No long incubations are required.
• Versatile: Binding/wash and elution conditions can be further optimized for individual polyhistidine- or HQ-tagged proteins.

Applications

• Expression and purification of polyhistidine-tagged proteins in rabbit reticulocyte lysate with less than 1% hemoglobin contamination.

For more information, see the Protocols & Applications Guide.

Notes

This product requires the use of a magnetic stand.

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