Promega has developed a new homogeneous reporter assay, the Dual-Glo™ Luciferase Assay System, for monitoring both firefly and Renilla luciferases expressed in mammalian cells in culture medium in 96- and 384-well plates. The new assay system is similar to the Dual-Luciferase® Reporter Assay System, except that cell lysis is integrated into the luminescence chemistry and the luminescence kinetics have been extended over several hours. The homogeneous assay format allows dual-reporter assays to be more easily and rapidly performed in multiwell plates by reducing sample processing requirements and eliminating the need for reagent injectors in the luminometer.
Promega Notes 81, 22–26.
Erika Hawkins, Braeden Butler, Michael Beck, Michael O'Grady, Laurie Orr and Keith Wood