Reverse Transcription System Protocol

Literature # TB099

AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ isolated RNA. The Reverse Transcription System provides lot-tested reagents to efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes. A polyadenylated 1.2kb transcript is provided as a control template for the cDNA synthesis reaction. cDNA synthesized with the Reverse Transcription System can be used directly in PCR.