Caspase-Glo® 9 Assay Technical Bulletin

Literature # TB333

The Caspase-Glo® 9 Assay is a homogeneous luminescent assay that measures caspase-9 activity. The assay provides a luminogenic caspase-9 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. Addition of a single Caspase-GloGlo® 9 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a "glow-type" luminescent signal (produced by the luciferase reaction). The signal generated is proportional to the amount of caspase activity present.