HisLink™ 96 Protein Purification System Technical Bulletin

Literature # TB342

The HisLink™ 96 Protein Purification System provides a simple, quick and robust method of purifying multiple polyhistidine- or HQ-tagged expressed proteins from E. coli using a vacuum-based method. The system is designed to purify expressed polyhistidine- or HQ-tagged proteins directly from deep-well, 96-well plates. The HisLink™ System is amenable to manual or automated methods, such as the Beckman Coulter Biomek® 2000 or FX for high-throughput applications.

In preparation for protein purification, bacterial cells expressing a polyhistidine- or HQ-tagged protein are lysed directly in culture using the provided FastBreak™ Cell Lysis Reagent. The HisLink™ resin is added directly to the lysate and mixed, and the polyhistidine- or HQ-tagged proteins bind in a matter of minutes.