ProtoBlot® II AP System with Stabilized Substrate Technical Manual

Literature # TM026

The ProtoBlot® II AP Systems with Stabilized Substrate improve upon the simplicity of the original ProtoBlot® Western Blot AP Systems by incorporating the premixed, ready-to-use Western Blue® Stabilized Substrate for Alkaline Phosphatase into a convenient, reliable immunodetection system. The ProtoBlot® II System is designed for the rapid, sensitive detection of proteins or other macromolecular antigens immobilized on nitrocellulose or polyvinylidene fluoride (PVDF) membranes, either transferred from gels after electrophoresis (Western blots) or bound directly from solution.