TNT® SP6 High-Yield Wheat Germ Protein Expression System Technical Manual

Literature # TM282

The TNT® SP6 High-Yield Wheat Germ Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system designed to express up to 100μg/ml of protein. This cell-free expression system is prepared from an optimized wheat germ extract and contains all the components (tRNA, ribosomes, amino acids, polymerase and translation initiation, elongation and termination factors) necessary for protein synthesis directly from DNA templates.
The TNT® SP6 High-Yield Wheat Germ Protein Expression System expresses genes cloned downstream of an SP6 RNA polymerase promoter.