Promega Corporation

Isolating Nucleic Acids from Small Amounts of Brain Lysates Using the SV Total RNA...

Isolating Nucleic Acids from Small Amounts of Brain Lysates Using the SV Total RNA Isolation System

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Abstract

The isolation of RNA from tissue and cellular sources is a necessary first step in a diverse array of molecular biological techniques. This report investigates the use of Promega's SV Total RNA Isolation System with decreasing amounts of rat total brain lysates. The amount of RNA isolated was linear over the range of tissue tested. Spectrophotometric analysis and the amplification of specific neural targets demonstrate the excellent quality and performance of the isolated RNA.

Promega Notes 69, 17.

Dan Kephart and Kyle Hooper

Promega Corporation
Publication Date: 1998

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