Promega Corporation

Automating Promega CellTiter-Glo Luminescent Cell Viability and Caspase-Glo 3/7 Assays...

Automating Promega CellTiter-Glo Luminescent Cell Viability and Caspase-Glo 3/7 Assays Scientific Poster

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Abstract

Tracy Worzella, M.S.1, Brad Larson, B.A.1, Michael Bjerke, M.S.1, Aoife Gallagher, Ph.D.2 and Eric Matthews, M.S.3
1Promega Corporation, Madison, WI, 2Deerac Fluidics, Dublin, Ireland, 3BMG LABTECH, Offenburg, Germany

We have demonstrated the scalability of the CellTiter-Glo® and Caspase-Glo® 3/7 assays for high-throughput cell-based screening in low-volume 384- and 1536-well formats. The Deerac Fluidics Equator™ NS 808 Eight Tip Low Volume Dispenser was used for non-contact delivery of sample and reagent. The multifunctional, PMT-based BMG LABTECH PHERAstar reader was used for signal detection. Excellent Z’-factor, %CV, and linear range data was obtained. Together, these scalable assays and instrumentation offer ideal tools for HTS users studying drug effects on cells during the drug discovery process.

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  • Part# PS020
  • Printed in USA.

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