Promega Corporation

Cell-Based Bioluminescent Hydrogen Peroxide Assay: Effects of Inducers and Generators...

Cell-Based Bioluminescent Hydrogen Peroxide Assay: Effects of Inducers and Generators with Cells Poster

Share

SEARCH POSTERS

Abstract

Hui Wang1, Jean Osterman1, Wenhui Zhou1, Poncho Meisenheimer1, John Shultz2, Sarah Duellman2, Jolanta Vidugiriene2, Gediminas Vidugiris2 and James Cali2
1 Promega Biosciences, Inc. 277 Granada Drive, San Luis Obispo, CA 93401
2 Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711

Consideration of the chemistry and biology of H2O2in cell culture:
• H2O2 has a relatively long half-life in solution and can diffuse in/out of the cell.
• Some compounds are easily oxidized in solution by air to produce H2O2 through a cell-independent mechanism.
• Several enzymes in the cell (e.g., monoamine oxidases (MAOs), D-amino acid oxidase, demethylases, etc.)directly produce H2O2 as a byproduct of their enzymatic reaction.
• Compounds, such as menadione and rotenone, that interfere with the electron transport chain of the mitochondria can induce H2O2 generation through the action of superoxide dismutase.
• Considerations important in determining a detection method for H2O2
• Ease of use
• Minimal false positive hits in the presence of library test compounds
• Adequate detection sensitivity
• Appropriate pH, temperature and buffer

Document Icon Download the poster PDF (472kb)

  • Part# PS174
  • Printed in USA.

Prefer a different language?

Your country is set to France. Your language is set to français. Please select the language that will best suit your needs:

This is correct, continue to site »

I need additional help

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.