Rapid Antibody Purification and Drug Conjugation Using High Capacity Magne™ Protein A...

Abstract

Nidhi Nath, Becky Godat, Marjeta Urh
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711.

We have developed high capacity magnetic Protein A and magnetic Protein G beads by combining two novel technologies.

1. Use of magnetic beads based on high surface area macroporous cellulose.
2. Covalent and oriented immobilization of Protein A and Protein G on the magnetic bead using HaloTag® Technology.

Magne™ Protein A Beads and Magne™ Protein G Beads purify polyclonal and monoclonal antibodies from serum, ascites fluid and cell media. High capacity magnetic Protein G and Protein A beads can be used to conjugate antibodies with fluorescent dyes and small molecules for ADC applications. Advantages of on-bead antibody conjugation includes, no need of purified antibodies, labeling through thiol or amine groups, efficient labeling of dilute antibodies, no dialysis or buffer exchange is required and parallel labeling of up to 96 samples.