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Nucl. Acids Res. 33, 4762-4774. Characterization of SpPol4, a unique X-family DNA polymerase in Schizosaccharomyces pombe. 2005

Gonzalez-Barrera, S., Sanchez, A., Ruiz, J.F., Juarez, R., Picher, A.J., Terrados, G., Andrade, P. and Blanco, L.

Notes: An open reading frame encoding a putative DNA polymerase, SpPol4, was amplified from S. pombe genomic DNA by PCR. The resulting PCR product was cloned into the pGEM®-T Easy Vector, and the sequence was verified. Based on sequence analysis, the authors hypothesize that SpPol4 has deoxyribose phosphate (dRP) lyase activity, suggesting that the enzyme plays a role in base excision repair. The authors perform a dRP lyase activity assay with an oligonucleotide substrate labeled using [32P]ddATP and terminal deoxynucleotidyl transferase. (3465)

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Biochem. Biophys. Res. Commun. 309, 222–231. Hypoxia induces apoptosis in SV40-immortalized rat proximal tubular cells through the mitochondrial pathways, devoid of HIF1-mediated upregulation of Bax. 2003

Tanaka, T., Hanafusa, N., Ingelfinger, J.R., Ohse, T., Fujita, T., and Nangaku, M.

Notes: ImProm-II™ Reverse Transcriptase was used in real time RT-PCR to measure the ratio of Bax to Bcl-2 in immortalized rat proximal tubular cells (IRPTCs) cultured under normoxic or hypoxic conditions. The researchers used 1μg of total RNA in the reverse transcription reaction. Qiagen’s QuantiTest CYBR Green PCT Kit was used to quantify PCR products. Promega’s terminal deoxynucleotidyl transferase (TdT) was also used for TdT-mediated dUTP nick end labeling (TUNEL) assays on the cells. The TUNEL-stained cells were analyzed by FACS analysis. Data from these experiments was expressed as percent apoptotic cells.  (2849)

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Nat. Biotechnol. 21(10), 1233-1237. Large-scale genotyping of complex DNA. 2003

Kennedy, G.C., Matsuzaki, H., Dong, S., Liu, W.M., Huang, J., Liu, G., Su, X., Cao, M., Chen, W., Zhang, J., Liu, W., Yang, G., Di, X., Ryder, T., He, Z., Surti, U., Phillips, M.S., Boyce-Jacino, M.T., Fodor, S.P. and Jones, K.W.

Notes: Terminal Deoxynucleotidyl Transferase was used to biotin end-label PCR products generated from adaptor-ligated genomic DNA fragment templates. The labeled probes were then hybridized to microarrays spotted with SNP alleles. The Terminal Deoxynucleotidyl Transferase reaction was performed for 4 hours at 37°C using 15-20 units TdT, TdT reaction buffer and 18μM biotin-ddATP. (2758)

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J. Biol. Chem. 275, 8772-8778. Chaperones Hsp70 and Hsp40 suppress aggregate formation and apoptosis in cultured neuronal cells expressing truncated androgen receptor protein with expanded polyglutamine tract. 2000

Kobayashi, Y., Kume, A., Li, M., Doyu, M., Hata, M., Ohtsuka, K., Sobue, G.

Notes: The DeadEnd™ Colorimetric Apoptosis Detection System was used to detect apoptosis in transfected Neuro 2a cells. The cells were transfected with truncated androgen receptors that aggregate in either the nuclei or the cytoplasm. The cells with nuclear aggregates were apoptotic and those with cytoplasmic aggregates were mostly nonapoptotic. The location of the aggregates was determined with GFP fusions. The basic protocol of the DeadEnd™ Colorimetric Apoptosis Detection System was modified to use streptavidin-Texas Red® for fluorometric detection rather than colorimetric detection. Good detail is provided for the modifications. Neuro 2a cells expressing the aggregating AR mutations as well as various chaperonins were analyzed for cell viability at 24, 48 and 72 hours with the CellTiter 96® AQueous One Solution (MTS/PES). The name of the DeadEnd™ Colorimetric Apoptosis Detection System has been changed to DeadEnd™ Colorimetric TUNEL System. (0913)

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Proc. Natl. Acad. Sci. USA 97, 2668-2673. Defective embryonic neurogenesis in Ku-deficient but not DNA-dependent protein kinase catalytic subunit-deficient mice. 2000

Gu, Y., Sekiguchi, J., Gao, Y., Dikkes, P., Frank, K., Ferguson, D., Hasty, P., Chun, J., Alt, F.W.

Notes: The DeadEnd™ Colorimetric Apoptosis Detection System was used to look at apoptotic cells in wildtype and transgenic mouse spinal cord sections. The tissue was fixed in 4% paraformaldehyde, paraffin embedded and 5µm sections prepared. The DeadEnd™ Colorimetric Apoptosis Detection System is now called the DeadEnd™ Colorimetric TUNEL System. (1082)

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FASEB J. 13, 395-410.. A new role for neurotrophins: Involvement of brain-derived neurotrophic factor and neurotrophin-4 in hair cycle control. 1999

Botchkarev, V.A., Botchkarev, N.V., Welker, P., Metz, M., Lewin, G.R., Subramaniam, A., Bulfone-Paus, S., Hagen, E., Braun, A., Lommatzsch, M., Renz, H. and Paus, R.

Notes: Anti-Human BDNF pAb and Anti-Human NT-4 pAb were used for fluorescent immunohistochemical localization of the respective factors to hair follicles of mice. Cryostat sections (8µm thickness) were probed with 1:50 dilutions of the antibodies. Serial sections were also probed with end-labeled probes for BDNF mRNA and TrkB mRNA. The ISH probes were produced using Terminal Deoxynucleotidyl Transferase. (1432)

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Proc. Natl. Acad. Sci. USA 95, 15803-15808. Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord. 1998

Coulouarn, Y. , Lihrmann, I. , Jegou, S. , Anouar, Y. , Tostivint, H. , Beauvillain, J. C. , Conlon, J. M. , Bern, H. A. , Vaudry, H.

Notes: In this paper, Tfl DNA polymerase was used for two-step RT-PCR. The amplified fragments were cloned into the pGEM®-T Vector. Promega's Terminal Deoxynucleotidyl Transferase (TdT) was used to end label specific oligos for screening a frog brain cDNA library. T7 and T3 RNA polymerases were used to make digoxigenin labeled riboprobes for in situ hybridization. (1281)

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Proc. Natl. Acad. Sci. USA 95, 14349-14354. Inhibition of intrathymic T cell development by expression of a transgenic antagonist peptide. 1998

Levelt, C.N. , Mizoguchi, E. , Huang, X. , Zacks, R. , Bhan, A.K. , Tonegawa, S.

Notes: The authors used Promega's Terminal Deoxynucleotidyl Transferase (TdT) in a home-brew TUNEL assay. (0786)

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J. Immunol. 161, 897-908. T cell-mediated, IFN-gamma-facilitated rejection of murine B16 melanomas. 1998

Bohm, W., Thoma, S., Leithauser, F., Moller, P., Schirmbeck, R. and Reimann, J.

Notes: The authors cloned the Hepatitis B surface antigen coding region into the pCI Mammalian Expression Vector for expression in COS-7 cells. They also used Promega's Terminal Deoxynucleotidyl Transferase (TdT) in a home brew TUNEL assay. (1421)

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Proc. Natl. Acad. Sci. USA 94, 1338-1343. Antigen therapy eliminates T cell inflammation by apoptosis: Effective treatment of experimental autoimmune neuritis with recombinant myelin protein P2. 1997

Weishaupt, A., Gold, R., Gaupp, S., Giegerich, G., Hartung, H.-P. and Toyka, K.V.

Notes: TUNEL assays were performed on paraffin-embedded tissues that had undergone experimental autoimmune neuritis. Digoxigenin-labeled nucleotides were used with Promega's TdT enzyme. (1535)

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Cell 88, 503-513. Nonhomologous RNA recombination in a cell-free system: Evidence for a transesterification mechanism guided by secondary structure. 1997

Chetverin, A., Chetverina1, H., Demidenko, A. and Ugarov, V.

Notes: These authors used Promega's AMV Reverse Transcriptase and Terminal Deoxynucleotidyl Transferase in this paper. (2032)

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J. Exp. Med. 186, 1749-1756. Suppressive role of B cells in chronic colitis of T cell receptor alpha mutant mice. 1997

Mizoguchi, A. , Mizoguchi, E. , Smith, R. N. , Preffer, F. I. , Bhan, A. K.

Notes: The Terminal Deoxynucleotidyl Transferase (TdT) enzyme was used to label the fragmented DNA in frozen sections. The DNA was labeled with biotin-dUTP for the TUNEL Assay. (0652)

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EMBO J. 16, 2282-2293.. T-cell receptor ligation by peptide/MHC induces activation of a caspase in immature thymocytes: the molecular basis of negative selection. 1997

Clayton, L. K. , Ghendler, Y. , Mizoguchi, E. , Patch, R. J. , Ocain, T. D. , Orth, K. , Bhan, A. K. , Dixit, V. M. , Reinherz, E. L.

Notes: Terminal Deoxynucleotidyl Transferase (TdT) was used with biotinylated dUTP for immunohistological TUNEL assays of frozen sections of mouse fetal thymus tissue. (1309)

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J. Cell Biol. 135(4), 1085-1096. Adenovirus-mediated gene transfer of the tumor suppressor, p53, induces apoptosis in postmitotic neurons. 1996

Slack, R.S., Belliveau, D.J., Rosenberg, M., Atwal, J., Lochmuller, H., Aloyz, R., Haghighi, A., Lach, B., Seth, P., Cooper, E., Miller, F.D.

Notes: The MTT-based CellTiter 96® System was used to measure survival of rat primary sympathetic neurons in response to infection with varying titers of adenovirus or HSV-1. TUNEL staining using Promega's TdT also is shown. (2950)

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Development 122, 121-129. Apoptosis in the developing tooth: Association with an embryonic signaling center and suppression by EGF and FGF-4. 1996

Vaahtokari, A., Åberg, T., Thesleff, I.

Notes: Paraffin-embedded tissues were deparaffinized and treated with proteinase K. The sections were fixed in paraformaldehyde and labeled with digoxigenin-labeled dUTP. Histochemical detection was accomplished with an AP-conjugated antibody and BCIP/NBT staining. As a positive control, a section was treated with RQ1 RNase-Free DNase prior to labeling with Promega's TdT and the labeled-dUTP. (0211)

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