Krenke, B.E., Tereba, A., Anderson, S.J., Buel, E., Culhane, S., Finis, C.J., Tomsey, C.S., Zachetti, J.M., Masibay, A., Rabbach, D.R., Amiott, E.A. and Sprecher, C.J.
Notes: In this paper, researchers from both Promega and state crime laboratories provide an evaluation of the Powerplex® 16 System loci (CODIS, Penta D, Penta E, and amelogenin). Data from several laboratories using a variety of thermal cyclers and the ABI PRISM® 310 Genetic Analyzer or ABI PRISM® 377 DNA Sequencer are presented. The Powerplex® 16 System was able to consistently genotype samples from as little as 0.0625-2ng of genomic DNA template. Other factors such as reaction volume, annealing temperature, titration of AmpliTaq Gold® DNA Polymerase, magnesium, or primer pairs, and 1ng DNA mixtures were analyzed using the Powerplex® 16 System. The researchers also present data on stutter analysis and cross reactivity of primer sets on primate DNA templates for each loci. (2675)