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Qu’est-ce que le NanoBRET™

Le NanoBRET™ est une nouvelle technologie de BRET utilisant deux nouveaux partenaires : 
- la  luciférase Nanoluc® (donneur bioluminescent)
- la protéine Halotag® complexée au fluorophore NanoBRET™ 618 (accepteur fluorescent)

Comparé au BRET classique, le NanoBRET™ détecte des interactions de très faible niveau entre deux protéines d’intérêt.

L’éloignement des spectres donneur et accepteur ainsi que la forte bioluminescence de la Nanoluc ® permettent une amélioration de la gamme dynamique.

Le NanoBRET™ permet également de visualiser en cellules vivantes l’intéraction protéine-protéine in situ.

 

Pour plus d’informations :

Why is NanoLuc Better For BRET?

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