ImProm-II™ Reverse Transcriptase
Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages
Catalog Number:
Size
Catalog Number: A3801
Catalog Number: A3802
Catalog Number: A3803
cDNA Synthesis for the Analysis of Rare or Long Messages
ImProm-II™ Reverse Transcriptase enables robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The ImProm-II™ Reverse Transcriptase can be used to reverse transcribe total RNA, poly(A)+ mRNA or synthetic transcript RNA templates.
Advantages- Full-Length RT-PCR: Reverse transcribe RNA templates up to 8.9kb.
- Microarray-Compatible: Incorporate regular and Cy®3- and Cy®5-modified nucleotides.
- Scalable and Flexible: Reaction volumes of 1–20μl can be used in subsequent PCR, and the optimized buffer also allows for coupled RT-PCR.
- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
- Versatile: Use with your thermostable polymerase of choice for two-step RT-PCR.
- Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
cDNA Synthesis for Analysis of Rare or Long Messages
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
|
ImProm-II™ 5X Reaction Buffer |
M289B | 1 × 100μl | ||
|
ImProm-II™ Reverse Transcriptase |
M314B | 1 × 10μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 1 × 1.2ml | 25mM | View Product |
|
ImProm-II™ 5X Reaction Buffer |
M289A | 1 × 600μl | ||
|
ImProm-II™ Reverse Transcriptase |
M314A | 1 × 100μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 3 × 1.2ml | 25mM | View Product |
|
ImProm-II™ 5X Reaction Buffer |
M289C | 2 × 1,250μl | ||
|
ImProm-II™ Reverse Transcriptase |
M314C | 1 × 500μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
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