NanoBRET® Target Engagement K192 Kinase Selectivity System
Profile Selectivity Across a Diverse Kinase Panel in Live Cells
- Profile compound cellular selectivity against a panel of 192 human kinases, broadly representing the kinome
- Optimized, addition-only cellular protocol provides quantitative compound occupancy data for 192 full-length kinases in a single experiment
- K192 System provides the transfection-ready, arrayed K192 Kinase Vector Panel and a single NanoBRET® TE Kinase Assay
Catalog Number:
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Catalog Number: NP4050
Catalog Number: NP4060
Catalog Number: NP4100
Catalog Number: NP4101
Profile Compound Selectivity Against 192 Kinases in Live Cells
The NanoBRET® Target Engagement (TE) K192 Kinase Selectivity System can quantitatively measure intracellular compound occupancy against a panel of 192 kinases in a single experiment. The system relies on the NanoBRET® TE technology, a bioluminescence resonance energy transfer (BRET) method that uses the small, extremely bright NanoLuc® luciferase as the energy donor and a cell-permeable NanoBRET® TE Tracer as the energy acceptor. The K192 Kinase Selectivity System is a live-cell method using an addition-only, multiwell plate format with a single NanoBRET® TE Kinase Assay. The NanoBRET® TE K192 Kinase Selectivity System is optimized for each kinase to provide quantitative occupancy data, enabling cellular profiling of compound-kinase interactions.
NanoBRET® Target Engagement Assay Principle
The NanoBRET® TE K192 Kinase Selectivity System uses the NanoBRET® TE technology, a bioluminescence resonance energy transfer (BRET) method. In this method, a target is expressed in mammalian cells as a target-NanoLuc® fusion protein. BRET is achieved by luminescent energy transfer from NanoLuc® luciferase to a cell-permeable NanoBRET® TE Tracer that is bound to the target-NanoLuc® fusion protein. A test compound that is cell-permeable and competes with the tracer for target binding will result in a loss of NanoBRET® signal. Using the optimized assay conditions provided for each target, the assay yields quantitative intracellular compound affinity and occupancy. Compound occupancy is quantified using controls that allow the BRET signal to be normalized.
Customer Success Stories
Case Study: NanoBRET® TE for live cell profiling of a compound's selectivity across a panel of 192 full-length kinases.
Dr. Martin Matzuk explains the importance of NanoBRET® TE in their research.
Case Study: Single-plate kinome screening in live-cells to enable highly cost-efficient kinase inhibitor profiling.
See how the NanoBRET® TE K192 Kinase Selectivity System has been optimized for a 384-well plate format, allowing for higher throughput and more efficient compound screening per kit.
K192 Kinase Selectivity System Provides Turn-Key Cellular Profiling
The NanoBRET® TE K192 Kinase Selectivity System contains a NanoBRET® TE K192 Kinase Vector Panel and a single NanoBRET® TE Intracellular Kinase Assay (K-10). The optimized protocol offers an addition-only workflow using multiwell plates. Begin by simply transfecting the vector panel into HEK293 cells or TransfectNow™ HEK293 Cells in a multiwell plate. Following overnight incubation, add your test compounds to the assay plate and perform the NanoBRET® TE Kinase Assay (K-10), according to the optimized protocol provided. A mutimode plate reader is used to measure BRET.
K192 Kinase Vector Panel Simplifies Cellular Kinase Selectivity Profiling
- The NanoBRET® TE K192 Kinase Vector Panel contains 192 kinase-NanoLuc® fusion vectors that are broadly spread across the kinome (green dots on dendrogram).
- The NanoBRET® TE K192 Kinase Vector Panel is supplied in two 96-well plates (A and B).
- Each well of the K192 Kinase Vector Panel has a unique mix of two vectors—one kinase-NanoLuc® fusion vector and either carrier DNA or a regulatory protein expression vector for optimal transfections.
- The addition-only microplate workflow uses a pre-arrayed transfection-ready Kinase Vector Panel and a single NanoBRET® TE Kinase Assay.
Diverse Panel of 192 Kinases to Represent the Kinome
The 192 Kinases in the NanoBRET® TE K192 Kinase Selectivity System are represented as green dots in the dendrogram, showing that the system broadly covers the kinome.
Kinases Present in the K192 Panel
Note: A name with a forward slash "/" indicates the kinase and the regulatory genes are both present in the vector mix and will be co-expressed when transfected. For example, “CDK/CCNB1” in the table indicates CDK1 is co-expressed with CCNB1.
Get The Live-Cell Kinase Selectivity Profiling Data You Need
Cell-free biochemical techniques for measuring binding or activity of kinase inhibitors can fail to accurately predict engagement of kinases inside a cell.
The dendrograms compare the target occupancy obtained with 1µM crizotinib using the live-cell NanoBRET® TE K192 Kinase Selectivity System versus a biochemical cell-free approach. The results showed an improved spectrum of activity in cells, with only 16 targets engaged by crizotinib at 50% occupancy or greater using the NanoBRET® TE method, compared to 49 kinases engaged using the biochemical approach.
The 16 kinases that bound with >50% occupancy using the K192 Kinase Selectivity System are tabularized along with the mean occupancy obtained from three independent experiments. The standard deviations for these measurements indicates that the K192 Kinase Selectivity System is reproducible.
NanoBRET® TE Cellular
Crizotinib, 16 hits
Biochemical
Crizotinib, 49 hits
NanoBRET® TE K192 Kinase Selectivity Results for 1µM Crizotinib
Need additional kinases for your selectivity profiling?
Our Tailored R&D Solutions team provides Kinase Selectivity Profiling Services with an expanded kinase panel, in addition to the K192 Panel.
Featured Publications
The Cell Chemical Biology publication is the original demonstration of how NanoBRET® TE could be used for broad kinase selectivity profiling in live cells. This publication illustrates the first live-cell method to quantitatively profile compound occupancy against hundreds of kinases.
The STAR Protocols publication improves upon the Cell Chemical Biology work and is the basis for the NanoBRET® TE K192 Kinase Selectivity System. The authors show how a single NanoBRET® TE Kinase Assay can be used to profile compound engagement across 192 full-length kinases in a single live-cell experiment using a simple workflow.
NanoBRET® K192 TE Assay Analysis Tools
Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157D | 1 × 3.3ml | View Product | |
Tracer Dilution Buffer |
N219B | 1 × 50ml | View Product | |
Extracellular NanoLuc® Inhibitor |
N235C | 1 × 1.1ml | View Product | |
NanoBRET® Tracer K-10 |
N264B | 10 × 300μl | 400μM | |
NanoBRET® TE K192 Vector Plate A |
NP401A | 2 × 1 each | ||
NanoBRET® TE K192 Vector Plate B |
NP402A | 2 × 1 each | ||
Adhesive Foil Plate Seals |
NP4000 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE Tracers V2 Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(a) use NanoLuc®-branded luciferase or derivatives, or subunits of the NanoBiT® technology for all energy transfer determinations conducted with this product; and
(b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(c) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. No. 10,024,862; Japanese Pat. No. 7676487; and other patents and patents pending.
U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.
What's in the box
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157D | 1 × 3.3ml | View Product | |
Tracer Dilution Buffer |
N219B | 1 × 50ml | View Product | |
Extracellular NanoLuc® Inhibitor |
N235C | 1 × 1.1ml | View Product | |
NanoBRET® Tracer K-10 |
N264B | 10 × 300μl | 400μM | |
Transfection Carrier DNA |
E488B | 1 × 100μg | View Product | |
| pNL1.1.CMV[Nluc/CMV] Vector | N109A | 1 × 20μg | View Product | |
NanoLuc®-HIPK2 Fusion Vector |
NV322A | 1 × 20μg | 1μg/μl | View Product |
NanoBRET® TE K192 Vector Plate A |
NP401A | 2 × 1 each | ||
NanoBRET® TE K192 Vector Plate B |
NP402A | 2 × 1 each | ||
Adhesive Foil Plate Seals |
NP4000 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE Tracers V2 Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(a) use NanoLuc®-branded luciferase or derivatives, or subunits of the NanoBiT® technology for all energy transfer determinations conducted with this product; and
(b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(c) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoLuc® ORF TE Vector Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.
In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. No. 10,024,862; Japanese Pat. No. 7676487; and other patents and patents pending.
U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.
What's in the box
| Item | Part # | Size |
|---|---|---|
NanoBRET® TE K192 Vector Plate A |
NP401A | 2 × 1 each |
NanoBRET® TE K192 Vector Plate B |
NP402A | 2 × 1 each |
Adhesive Foil Plate Seals |
NP4000 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
What's in the box
| Item | Part # | Size |
|---|---|---|
NanoBRET® TE K192 Vector Plate A |
NP401A | 1 × 1 each |
NanoBRET® TE K192 Vector Plate B |
NP402A | 1 × 1 each |
Adhesive Foil Plate Seals |
NP4000 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
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