Maxwell® HT DNA FFPE Isolation System
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Ready-to-Go FFPE DNA Kit, Expert Support Available
The Maxwell® HT DNA FFPE Isolation System is compatible with automation on all major platforms, including Hamilton Star/Nimbus, Tecan Freedom Evo/Fluent, Biomek i5/i7, Eppendorf epMotion, and particle movers such as KingFisher.
With decades of experience in building personalized automation solutions, we can help you customize the scale, application, and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.
Rapid Automated Extraction of FFPE DNA on Liquid Handlers
- Simple, automation-friendly method
- Not centrifugation or vacuum
- No organic solvents
The Maxwell® HT DNA FFPE Isolation System provides a simple and reliable method for high-throughput, rapid isolation of genomic DNA from FFPE (formalin-fixed, paraffin-embedded) tissue samples. The purified DNA can be used directly in a variety of downstream applications, including PCR and next-generation sequencing.
The Maxwell® HT DNA FFPE Isolation System purifies nucleic acid using paramagnetic particles, which provide a mobile solid phase to optimize sample capture, washing and purification of gDNA.
FFPE System Performance Comparison
To demonstrate the performance of this system, Maxwell® HT DNA FFPE purification chemistry was automated on the Tecan Freedom EVO® and ThermoFisher KingFisher™ Flex instruments. Qiagen QIAamp® and GeneRead kits were run using a standard method on a QIAcube instrument. Consecutive FFPE sections of human lung tumor and normal adjacent tissue (NAT) were interspersed among all methods and replicates to minimize sample-based variability. Eluates were quantitated using fluorometric detection and quality was assessed using the ProNex® DNA QC Assay.
| Tissue | Instrument | Uniformity of Coverage (%) | Mean Amplicon Coverage (X) | Reads (millions) |
|---|---|---|---|---|
| Tumor | Tecan | 100.0 | 4818 | 1.08 |
| KingFisher | 99.19 | 6036 | 1.28 | |
| Normal Adjacent | Tecan | 100.0 | 5202 | 1.18 |
| KingFisher | 100.0 | 4074 | 1.05 |
Next-Generation Sequencing Performance of DNA Purified with Maxwell® HT Chemistry. DNA purified using Maxwell® HT chemistry on both Tecan and KingFisher instruments was sequenced using an Illumina MiSeq after enrichment using the Ion AmpliSeq™ Cancer Hotspot Panel v2. Coverage, uniformity and depth were extremely high for both methods and for both tissue types, showing the suitability of these eluates in NGS applications. Furthermore, subsequent variant analysis revealed that low-frequency variants in the KRAS and EGFR genes were detected in the tumor tissue samples, but not in the normal tissue samples.
Automated DNA Extraction: FFPE Data
These application notes contain protocols and example data using the Maxwell® HT DNA FFPE Isolation System to purify DNA from formalin-fixed, paraffin-embedded tissue samples using the Kingfisher™ Flex system.
Your Purification Solution, Simplified
Our team of automation experts will help you choose an optimized high-throughput solution that meets your laboratory’s needs. We can implement Maxwell® HT chemistries on a variety of sample types and instrumentation platforms. Contact us for a consultation about choosing the right purification chemistry or customizing a purification chemistry for additional sample types.
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