Mass Spec-Compatible Yeast and Human Protein Extracts
Protein Extracts for Easier Mass Spec Instrument Monitoring
- Choose human or yeast, digested or intact extract
- Monitor LC and MS instrument performance
- Use for method development and optimization
Catalog Number:
Choose a Species
Size
Catalog Number: V7461
Catalog Number: V7341
Catalog Number: V6951
Catalog Number: V6941
Yeast/Human Protein Extracts for Monitoring LC/MS
The Mass Spec-Compatible Yeast and Human Protein Extracts are designed for monitoring mass spec instrument performance. The protein extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC-MS) analysis.
Each extract is available in a pre-digested, tryptic peptide form, used primarily for monitoring mass spectrometry instrument performance. The yeast and human extracts are also offered in an intact undigested form to provide a test material for optimizing mass spec protein sample preparation.
Extract reproducibility is assured by stringent control over the manufacturing process. Each protein extract is monitored by LC-MS to confirm composition and protein integrity.
LC/MS Performance: Consistent Mass Spec Profiles
A Model System for Method Development
These protein extracts provide ideal model systems for development and optimization of various sample prep methods, including isobaric tagging and enrichment.
In the example of isobaric tagging shown here, three aliquots of the human K562 predigested extract were labeled with three different TMT isobaric mass tag reagents (TMT126, TMT127 and TMT128) and mixed at a 10:5:1 ratio. Relative quantitative ratio between the mass tags was determined with an LTQ Orbitrap Velos Pro™ mass spectrometer. The data show good correlation between the expected and observed protein quantitation levels.
Enhanced Quality Control Ensures Reliable, Reproducible Performance
| Quality Measurement | MS-Compatible Human Protein Extract Digest (Cat.# V6951) | HeLa Protein Digest Standard (Other Supplier) |
|---|---|---|
|
Missed Cleavages
|
<10%
|
<10%
|
|
Peptide Quality |
Quantification of amino acids in sample Protein fragmentation: 1% or less Matching spectra: >65%
|
A280 Not tested; no specification Not tested; no specification
|
|
Lot-to-Lot Consistency
|
>12,462 unique peptides Total proteins: >1805 Protein identification: >85% of 1,194 human core peptides Quantitation reproducibility monitored by relative abundance of 10 reference proteins |
LC-MS chromatogram conforms to reference - Ratio of peptide area to reference = 0.75–1.125 Not tested |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Size |
|---|---|---|
MS Compatible Yeast Protein Extract, Digest |
V746A | 1 × 100μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box
| Item | Part # | Size | Concentration |
|---|---|---|---|
MS Compatible Yeast Protein Extract, Intact |
V734A | 1 × 1mg | 10mg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box
| Item | Part # | Size |
|---|---|---|
MS Compatible Human Protein Extract, Digest |
V695A | 1 × 100μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box
| Item | Part # | Size | Concentration |
|---|---|---|---|
MS Compatible Human Protein Extract, Intact |
V694A | 1 × 1mg | 10mg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
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