rLys-C, Mass Spec Grade
Recombinant Lys-C Cleaves at C-Terminus of Lysines
- Optimal activity in the pH range of 8–9
- Retains activity in denaturing conditions such as 8M urea
- Supplied lyophilized
Catalog Number:
Size
Catalog Number: V1671
rLys-C, Mass Spec Grade, is a recombinant Lys-C expressed in E. coli. Sequence origin of rLys-C is Protease IV from Pseudomonas aeruginosa. Similar to a native Lys-C, rLys-C cleaves at the carboxyl side of lysine residues with exceptional specificity. rLys-C retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. rLys-C activity is optimal in the pH range of 8–9. The protease is supplied in a lyophilized form along with a Reconstitution Buffer, which is formulated to increase stability of rLys-C solution. Lyophilized rLys-C solution can be stored for a month at –20ºC without detectable loss of activity. rLys-C is recommended for digestion of single proteins and complex protein mixtures in-solution and in-gel.
rLys-C has been shown to be free of contaminating peptides by reverse-phase HPLC. This recombinant product is severalfold less expensive than, yet retains the activity of native rLys-C.
Applications
- Peptide mapping
- Protein identification
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Size |
|---|---|---|
|
rLys-C, Mass Spec Grade |
V167A | 1 × 15μg |
|
Resuspension Buffer |
V181A | 1 × 500μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Related Products
Similar Products
rChymoSelect™ MS Grade Protease Kit
Recombinant chymotrypsin engineered for precise, reproducible digests in peptide mapping workflows.
CS333204, CS333205
Trypsin/Lys-C Mix, Mass Spec Grade
A mixture of Trypsin Gold, Mass Spec Grade, and rLys-C, Mass Spec Grade, designed to improve digestion of proteins or protein mixtures in solution.
V5071, V5072, V5073
rAsp-N, Mass Spec Grade
Cleavage site: N-terminal of Asp. Optimal pH: 6–9.
VA1160
Lys-C, Mass Spec Grade
Cleavage site: C-terminal of Lys. Optimal pH: 7–9.
VA1170
Endoglycosidase H
Characterize protein glycosylation in applications such as monitoring protein trafficking and determining glycan location and structure by mass spectrometry.
V4871, V4875
Fetuin
Glycoprotein with O-linked and N-linked glycosylation sites; Ideal control substrate to monitor PNGase F or Endo H activity.
V4961
Frequently Used With
Arg-C Ultra, Mass Spec Grade
Endopeptidase that cleaves at the C-terminus of arginine residues, including Arg-Pro.
VA1831, VA1832
Sequencing Grade Modified Trypsin
Trypsin manufactured for maximum specificity.
V5111, V5117, V5113
Trypsin Gold, Mass Spectrometry Grade
Maximum digest specificity with extreme resistance to autolytic digestion
V5280
ProteaseMAX™ Surfactant, Trypsin Enhancer
For improved in-gel digestion and protein solubilization.
V2071, V2072
