Trypsin Gold, Mass Spectrometry Grade
Maximum Trypsin Digest Specificity, Extreme Resistance to Autolytic Digestion
- Each lot qualified by mass spectrometry to ensure compatibility with your applications/instrumentation
- Chymotrypsin activity eliminated for distinct, consistent data
- Digest in gel or in solution
Catalog Number:
Size
Catalog Number: V5280
Excellent Specificity and Resistance to Autolytic Digestion
Trypsin Gold, Mass Spectrometry Grade, is manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized to yield Trypsin Gold, Mass Spectrometry Grade.
Each lot of quality-tested Trypsin Gold, Mass Spectrometry Grade, is qualified for use with in-gel protein digestion and mass spectrometric analysis. For more information, see the Protein Purification and Analysis chapter of the Protocols and Applications Guide.
See our newest trypsin: Rapid Digestion-Trypsin and Rapid Digestion-Trypsin/Lys-C
Learn more about our custom options for this product at: www.promega.com/custom/
Spectrogram Showing Digestion with Trypsin Gold, Mass Spec Grade
A 500ng sample of carbonic anhydrase II was separated by gel electrophoresis and digested with 500ng of Trypsin Gold, Mass Spectrometry Grade, overnight at 37°C.
Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Size |
|---|---|---|
|
Trypsin Gold, Mass Spectrometry Grade |
V528A | 1 × 100μg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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