Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo® Plus Assay
Tracy Worzella and Brad Larson
Promega Corporation
Publication Date: 2006
Promega Corporation
Publication Date: 2006
Abstract
Here we demonstrate the concept of kinase profiling using a panel of four kinases and ten kinase inhibitors. We used the Kinase-Glo® Plus Luminescent Kinase Assay and the Deerac Fluidics™ Equator™ HTS non-contact liquid handling system to design and perform an ultrahigh-throughput profiling screen. We present IC50 values for each test compound and show how such profiling can streamline the initial drug discovery process.
Promega Notes 93, 2–4.
Learn about the chemistry of the bioluminescent reaction and its use in a broad range of assays.
