CloneWeaver® Workflow Purchasing Tool

The PureYield Plasmid Maxiprep System isolates transfection-quality plasmid DNA. The system provides a rapid method for purification of up to 1mg of plasmid DNA from a 250ml bacterial culture. Plasmid DNA can be purified rapidly with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. The PureYield Plasmid Maxiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TNT Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA. The system has been designed for use with a vacuum source and vacuum manifold (e.g., the Vac-Man Laboratory Vacuum Manifold). The Eluator Vacuum Elution Device is used to elute nucleic acids from PureYield Midiprep or Maxiprep columns. It consists of two pieces, a blue base and a clear column assembly. The base interfaces with a Vacuum Manifold that contains Luer-Lok fittings, such as the Vac-Man Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield Midiprep or Maxiprep columns. The Eluator Device eliminates the requirement for a centrifuge with a swinging bucket rotor for nucleic acid purification, simplifying and speeding purification protocols.

The PureYield Plasmid Midiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify 100-200ug of plasmid DNA from a 50ml bacteria culture. Purify plasmid DNA in as little as 30 minutes with the vacuum protocol, greatly reducing time spent compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400ug of high-copy-number plasmid from 250ml of E. coli culture. The system incorporates a unique Endotoxin Removal Wash to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Purify without isopropanol precipitation or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).

The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15ug of plasmid DNA from 600ul to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TNT Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).

The Wizard MagneSil Plasmid DNA Purification System provides a simple and reliable method for the rapid isolation of plasmid DNA in a 96-well, high-throughput format. The purified plasmid can be used directly for automated fluorescent sequencing, such as with BigDye terminator sequencing chemistry, as well as for other standard molecular biology techniques including restriction enzyme digestion. The use of the MagneSil Paramagnetic Particles for lysate clearing (BLUE) as well as DNA capture (RED) circumvents the need for centrifugation or vacuum manifolds, making the system ideal for full automation on a Beckman Coulter or Tecan instrument.

The Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques, including restriction enzyme digestion. The Wizard SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.

The Wizard SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40ug of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

The x-tracta Gel Extractor tool provides a convenient, safe method for removal of agarose gel fragments for further processing. The device removes a 0.13 x 0.33 inch gel piece from agarose gels for easy transfer into a microcentrifuge tube for processing. The x-tracta tool eliminates the need for razor blades or scalpels, and its single-use design eliminates the possibility for sample-to-sample cross-contamination. Note: The x-tracta Gel Extractor works best on 0.6-2% analytical grade agarose gels. Please exercise caution if using the x-tracta Gel Extractor on Low Melting Point (LMP) agarose gels because the extractor does not work effectively due to the gel consistency.

FuGENE HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components. The cell lines listed in Table 1 of TM328 have been successfully transfected by Promega Corporation or Fugent, L.L.C. For a list of conditions that were used in the transfection of these and other cell types, visit our FuGENE HD Protocol Database at: www.promega.com/techserv/tools/FugeneHD/

Le réactif de transfection FuGENE 6 est une préparation non liposomale conçue pour transfecter l'ADN plasmidique dans une grande variété de lignées cellulaires avec un rendement élevé et une toxicité faible. Le protocole ne nécessite pas l'élimination du sérum ou du milieu de culture, et ne nécessite pas de lavage ou de changement de milieu après l'introduction du complexe réactif-ADN.

ViaFect est un réactif de transfection innovant conçu pour introduire efficacement l'ADN dans une grande variété de lignées cellulaires. Le réactif de transfection ViaFect a prouvé son efficacité dans les modèles de cellules adhérentes couramment utilisés ainsi que dans des lignées cellulaires traditionnellement considérées comme difficiles à transfecter, telles que les cellules en suspension et les lignées dérivées de cellules souches. Ce réactif doux à toxicité faible permet aux cellules de rester en bonne santé et métaboliquement actives lors des expériences de transfection, et propose un protocole simple d'emploi qui n'exige pas l'élimination du sérum ou du milieu de culture avant utilisation. Après l'introduction du complexe réactif-ADN, il n'est pas nécessaire de procéder à un lavage ou de modifier le milieu. Le réactif de transfection ViaFect garantit d'excellentes performances avec une optimisation minimale, permettant ainsi une conception simple des dosages les plus pertinents.